bsm-54188R [Primary Antibody]
Mad2L2 (1C4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Mad2L2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10459

Swiss Prot: Q9UI95

Source: Recombinant full length protein of human Mad2L2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T-47D, was shown to complete mitosis in the presence of Nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during pro-metaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with Insulin receptor (IR), but not IGF-IR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and, if the process is not complete, MAD2B delays the onset of anaphase.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 24


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SiHa Cells; Probed with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with Mad2L2 (1C4) Monoclonal Antibody (bsm-54188R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).