bsm-54191R [Primary Antibody]
Staufen (8C8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: STAU1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6780

Swiss Prot: O95793

Source: Recombinant protein within human Staufen aa 400-550

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

STAU1 (staufen, RNA binding protein, homolog 1) is a 577 amino acid protein that contains three double-stranded RNA-binding domains and is a mammalian homolog of Staufen, a Drosophila protein that is involved in mRNA transport during oogenesis and zygotic development. Localized to the rough endoplasmic reticulum (RER) and expressed in a variety of tissues, including heart, brain, liver, lung, pancreas, kidney and placenta, STAU1 binds to both Tubulin and double-stranded RNA and is thought to play an important role in mRNA transport from the microtubule network to the RER. Additionally, STAU1 may be involved in cross-linking cytoskeletal components with RNA, an event that is important for proper mRNA positioning during translation. Alternative splicing of the STAU1 gene yields two STAU1 isoforms, designated short and long.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

IF(ICC) staining with Staufen (8C8) Monoclonal Antibody (bsm-54191R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Staufen (8C8) Monoclonal Antibody (bsm-54191R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Staufen (8C8) Monoclonal Antibody (bsm-54191R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Lane 1: K562 Cells; Probed with Staufen (8C8) Monoclonal Antibody (bsm-54191R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of SH-SY5Y cells with Staufen (8C8) Monoclonal Antibody (bsm-54191R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).