bsm-54194R [Primary Antibody]
SPTAN1 (4C8) Monoclonal Antibody
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Host: Rabbit

Target Protein: SPTAN1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6709

Swiss Prot: Q13813

Source: Recombinant protein within human Alpha Fodrin aa 2300-2450

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. SPTAN1 is an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants. SPTAN1, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Predicted Molecular Weight: 285/150


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Hela Cells; Lane 2: Jurkat Cells; Lane 3: NIH-3T3 Cells; Lane 4: Rat Brain; Lane 5: Mouse Brain; Probed with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.