bsm-54198R [Primary Antibody]
TPT1 (7C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TPT1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7178

Swiss Prot: P13693

Source: Recombinant protein corresponding to human TCTP 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

TPT1 is a regulator of cellular growth and proliferation. Its mRNA is highly structured and contains an oligopyrimidine tract (5'-TOP) in its 5' untranslated region that functions to repress its translation under quiescent conditions. The protein is involved in a variety of cellular pathways, including apoptosis, protein synthesis and cell division. It binds to and stabilizes microtubules, and removal of this protein through phosphorylation is required for progression through mitotic and meiotic cell divisions. This gene is known to play a role in carcinogenesis, and is upregulated in some cancer cells. Alternative splicing results in multiple transcript variants encoding different isoforms.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 20


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Placenta; Lane 2: Mouse Testis; Lane 3: Hela Cells; Lane 4: A431 Cells; Lane 5: MCF-7 Cells; Probed with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of THP-1 cells with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).