VALIDATION IMAGES
Lane 1: Mouse Placenta; Lane 2: Mouse Testis; Lane 3: Hela Cells; Lane 4: A431 Cells; Lane 5: MCF-7 Cells; Probed with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of THP-1 cells with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).