VALIDATION IMAGES
IF(ICC) staining with THOC1 (7G3) Monoclonal Antibody (bsm-54202R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Lane 1: Hela Cells; Lane 2: A431 cells; Lane 3: Mouse Skeletal Muscle; Lane 4: PC-12 Cells; Probed with THOC1 (7G3) Monoclonal Antibody (bsm-54202R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with THOC1 (7G3) Monoclonal Antibody (bsm-54202R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with THOC1 (7G3) Monoclonal Antibody (bsm-54202R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with THOC1 (7G3) Monoclonal Antibody (bsm-54202R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with THOC1 (7G3) Monoclonal Antibody (bsm-54202R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsils tissue incubated with THOC1 (7G3) Monoclonal Antibody (bsm-54202R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with THOC1 (7G3) Monoclonal Antibody (bsm-54202R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Rat Placenta tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human HepG2 cell lysates probed with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human HUVEC cell lysates; Lane 5: Human LOVO cell lysates probed with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.