VALIDATION IMAGES
Lane 1: Hela Cells; Probed with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Cervix tissue incubated with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Hela cells with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).