DATASHEET
Host:
Rabbit
Target Protein:
IGFBP2
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
3485
Swiss Prot:
P18065
Source:
Recombinant protein within mouse IGFBP2 aa 1-250
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Background:
The Insulin-like growth factor-binding proteins (IGFBPs), a family of homologous proteins that have co-evolved with the IGFs, serve not only as shuttle molecules for the soluble IGFs, but also confer a level of regulation to the IGF signaling system. Physical association of the IGFBPs with IGF influences the bio-availability of the growth factors, and their concentration and distribution in the extracellular environment. The IGFBPs also appear to have biological activity independent of the IGFs. Seven IGFBPs have been described, each differing in their tissue distribution, half-lives and modulation of IGF interactions with their receptors. IGFBP1 is negatively regulated by Insulin production. The IGFBP1 gene is expressed at a high level during fetal liver development and in response to nutritional changes and diabetes. IGFBP2, which may function as a chaperone, escorting IGFs to their target tissues, is expressed in several human tissues including fetal eye and fetal brain.
VALIDATION IMAGES
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with IGFBP2 (12C6) Monoclonal Antibody (bsm-54213R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Human Serum; Lane 2: NCCIT Cells; Probed with IGFBP2 (12C6) Monoclonal Antibody (bsm-54213R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.