bsm-54220R [Primary Antibody]
GAB1 Recombinant Antibody
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Host: Rabbit

Target Protein: GAB1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 2549

Swiss Prot: Q13480

Source: Recombinant protein within human GAB1 aa 400-600

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

GAB1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Predicted Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

IF(ICC) staining with GAB1 (13C4) Monoclonal Antibody (bsm-54220R) at 1:200 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with GAB1 (13C4) Monoclonal Antibody (bsm-54220R) at 1:200 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with GAB1 (13C4) Monoclonal Antibody (bsm-54220R) at 1:200 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with GAB1 (13C4) Monoclonal Antibody (bsm-54220R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with GAB1 (13C4) Monoclonal Antibody (bsm-54220R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Hela cells with GAB1 (13C4) Monoclonal Antibody (bsm-54220R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


25 ug total protein per lane of various lysates (see on figure) probed with GAB1 monoclonal antibody, unconjugated (bsm-54220R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.