bsm-54228R [Primary Antibody]
SEC23A (3C6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SEC23A

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10484

Swiss Prot: Q15436

Source: Recombinant protein within human SEC23 aa 300-500

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

SEC23A is a member of the SEC23 subfamily of the SEC23/SEC24 family. It is part of a protein complex and found in the ribosome-free transitional face of the endoplasmic reticulum (ER) and associated vesicles. Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex. Required for the translocation of insulin-induced glucose transporter SLC2A4/GLUT4 to the cell membrane.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: NIH-3T3 Cells; Probed with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of HepG2 cells with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).