VALIDATION IMAGES
Lane 1: NIH-3T3 Cells; Probed with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of HepG2 cells with SEC23A (3C6) Monoclonal Antibody (bsm-54228R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).