bsm-54231R [Primary Antibody]
Coilin (1C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Coilin

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8161

Swiss Prot: P38432

Source: Recombinant protein within human Coilin aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Coilin protein is one of the main molecular components of Cajal bodies (CBs). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. In addition to its structural role, coilin acts as a glue to connect the CB to the nucleolus. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influence its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome and chromosome.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Rat Kidney; Probed with Coilin (1C1) Monoclonal Antibody (bsm-54231R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of Jurkat cells with Coilin (1C1) Monoclonal Antibody (bsm-54231R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).