bsm-54235R [Primary Antibody]
CRMP1 (6C5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CRMP1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1400

Swiss Prot: Q14194

Source: Recombinant protein within human CRMP1 aa 400 to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Collapsin response mediator proteins (CRMPs), including CRMP-1 (DRP-1), CRMP-2 (DRP-2 or TOAD64), CRMP-3 (DRP-4), CRMP-4 (DRP-3) and CRMP-5 (DRP-5), mediate signal transduction after exposure of neural cells to the axon guidance molecule Semaphorin 3A (SEMA3A)/collapsin. CRMPs are present in the developing cerebral cortex and neocortical neurons and are responsive to SEMA3A. In the adult brain, the expression of CRMPs is dramatically downregulated. However, they remain expressed in structures that retain their capacity for differentiation and plasticity. CRMP-1 is also involved in cancer invasion and metastasis. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. CRMP-1 plays a role in axon guidance, invasive growth and cell migration. CRMP-1 may participate in cytokinesis.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 74/62


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Brain; Probed with CRMP1 (6C5) Monoclonal Antibody (bsm-54235R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with CRMP1 (6C5) Monoclonal Antibody (bsm-54235R) at 1:100 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with CRMP1 (6C5) Monoclonal Antibody (bsm-54235R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with CRMP1 (6C5) Monoclonal Antibody (bsm-54235R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with CRMP1 (6C5) Monoclonal Antibody (bsm-54235R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with CRMP1 (6C5) Monoclonal Antibody (bsm-54235R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with CRMP1 (6C5) Monoclonal Antibody (bsm-54235R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of SH-SY5Y cells with CRMP1 (6C5) Monoclonal Antibody (bsm-54235R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).