VALIDATION IMAGES
Lane 1: Mouse Testis; Lane 2: SiHa Cells; Lane 3: SK-BR-3 Cells; Lane 4: PC-3M Cells; Probed with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of LOVO cells with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).