bsm-54245R [Primary Antibody]
Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PFKM

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5213

Swiss Prot: P08237

Source: Recombinant protein within human Fructose 6 Phosphate Kinase 1-200

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. In human PFK exists as a system of 3 types of subunits, PFKM (muscle), PFKL (liver) and PFKP (platelet) isoenzymes.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 85


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: PC-3M Cells; Probed with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Thyroid Gland tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of SH-SY5Y cells with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).