bsm-54250R [Primary Antibody]
SF3B1 (6C6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SF3B1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3451

Swiss Prot: O75533

Source: Recombinant protein within human SF3B1 aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 146


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Thymus; Lane 2: Hela Cells; Probed with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with SF3B1 (6C6) Monoclonal Antibody (bsm-54250R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.