VALIDATION IMAGES
Lane 1: Hela Cells; Lane 2: SiHa Cells; Probed with OGT (11F2) Monoclonal Antibody (bsm-54263R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with OGT (11F2) Monoclonal Antibody (bsm-54263R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with OGT (11F2) Monoclonal Antibody (bsm-54263R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with OGT (11F2) Monoclonal Antibody (bsm-54263R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with OGT (11F2) Monoclonal Antibody (bsm-54263R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with OGT (11F2) Monoclonal Antibody (bsm-54263R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with OGT (11F2) Monoclonal Antibody (bsm-54263R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with OGT (11F2) Monoclonal Antibody (bsm-54263R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Hela cells with OGT (11F2) Monoclonal Antibody (bsm-54263R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).