DATASHEET
Host:
Rabbit
Target Protein:
CMA1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
1215
Swiss Prot:
P23946
Source:
Recombinant protein within human Mast Cell Chymase aa 50-200
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Background:
Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Mast Cell Chymase, also known as CMA1 or MCT1, is a major secreted serine protease that is involved in vasoactive peptide generation, extracellular matrix degradation and regulation of gland secretion. The human chymase gene, which maps to human chromosome 14q11.2, encodes a preproenzyme with a 19 amino acid signal peptide, an acidic 2 amino acid propeptide and a 226 amino acid catalytic domain. Mast Cell Chymase is a chymotryptic serine proteinase which is a member of the peptidase family S1. Expressed in mast cells, Mast Cell Chymase is associated with the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. Mast cell proteases are a family of rodent protein homologs to human tryptases that are specifically expressed in mast cells and may serve as highly specific markers in the analysis of mast cell heterogeneity, differentiation and function.
VALIDATION IMAGES
Lane 1: Rat Spleen; Lane 2: Mouse Spleen; Probed with Mast Cell Chymase (1G11) Monoclonal Antibody (bsm-54265R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with Mast Cell Chymase (1G11) Monoclonal Antibody (bsm-54265R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Mast Cell Chymase (1G11) Monoclonal Antibody (bsm-54265R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.