bsm-54266R [Primary Antibody]
Wnt2b (2G5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Wnt2b

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7482

Swiss Prot: Q93097

Source: Recombinant protein within human Wnt2b aa 200-350

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lan Zhang. et al. Inhibited HDAC3 promotes microRNA-376c-3p to suppress malignant phenotypes of gastric cancer cells by reducing WNT2b. Genomics. 2021 JulRead more>>
VALIDATION IMAGES

Lane 1: SiHa Cells; Probed with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:200 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:200 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mosue Kidney tissue incubated with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of LOVO cells with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Testis tissue lysates; Lane 5: Human U-2os cell lysates; Lane 6: Human Huvec cell lysates; Lane 7: Human U-87 MG cell lysates; Lane 8: Human Hela cell lysates probed with Wnt2b Monoclonal Antibody, Unconjugated (bsm-54266R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.