VALIDATION IMAGES
Lane 1: SiHa Cells; Probed with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:200 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:200 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mosue Kidney tissue incubated with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of LOVO cells with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Testis tissue lysates; Lane 5: Human U-2os cell lysates; Lane 6: Human Huvec cell lysates; Lane 7: Human U-87 MG cell lysates; Lane 8: Human Hela cell lysates probed with Wnt2b Monoclonal Antibody, Unconjugated (bsm-54266R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.