bsm-54266R [Primary Antibody]
Wnt2b Recombinant Antibody
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Host: Rabbit

Target Protein: Wnt2b

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 7482

Swiss Prot: Q93097

Source: Recombinant protein within human Wnt2b aa 200-350

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF
Flow-Cyt(1ug/Test)
ICC/IF(1:50-100)

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lan Zhang. et al. Inhibited HDAC3 promotes microRNA-376c-3p to suppress malignant phenotypes of gastric cancer cells by reducing WNT2b. Genomics. 2021 JulRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mosue Kidney tissue incubated with Wnt2b (2G5) Monoclonal Antibody (bsm-54266R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


25 ug total protein per lane of various lysates (see on figure) probed with Wnt2b monoclonal antibody, unconjugated (bsm-54266R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.