bsm-54270R [Primary Antibody]
PSMB9 (1G9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PSMB9

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5698

Swiss Prot: P28065

Source: Synthetic peptide within human PSMB9 aa 180 to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 23


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Spleen; Lane 2: U937 Cells; Lane 3: Mouse Colon; Probed with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Daudi cells with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).