DATASHEET
Host:
Rabbit
Target Protein:
PSMB9
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
5698
Swiss Prot:
P28065
Source:
Synthetic peptide within human PSMB9 aa 180 to the C-terminus
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Background:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
VALIDATION IMAGES
Lane 1: Mouse Spleen; Lane 2: U937 Cells; Lane 3: Mouse Colon; Probed with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Daudi cells with PSMB9 (1G9) Monoclonal Antibody (bsm-54270R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).