VALIDATION IMAGES
Lane 1: Human Kidney; Lane 2: MCF-7 Cells; Probed with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of MCF-7 cells with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).