bsm-54275R [Primary Antibody]
PCK2 (12C5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PCK2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5106

Swiss Prot: Q16822

Source: Recombinant protein within C-terminal human PCK2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 71


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human Kidney; Lane 2: MCF-7 Cells; Probed with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of MCF-7 cells with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).