Host: Rabbit
Target Protein: HIF-2 alpha
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 2034
Swiss Prot: Q99814
Source: A synthesized peptide derived from human HIF 2 alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot dependent
Predicted Molecular Weight: 96
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Rat Testis tissue lysates ; Lane 5: Human HepG2 cell lysates; Lane 6:Human MCF-7 cell lysates; Lane 7: Human U87MG cell lysates; Lane 8: Human SH-SY5Y cell lysates probed with EPAS1 (2G3) Monoclonal Antibody, Unconjugated (bsm-54278R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with HIF-2 alpha monoclonal antibody, unconjugated (bsm-54278R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.