bsm-54280R [Primary Antibody]
NPHS2 (1A5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NPHS2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7827

Swiss Prot: Q9NP85

Source: Recombinant protein within C-terminal human NPHS2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Rat Kidney; Lane 2: Human Kidney; Probed with NPHS2 (1A5) Monoclonal Antibody (bsm-54280R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with NPHS2 (1A5) Monoclonal Antibody (bsm-54280R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with NPHS2 (1A5) Monoclonal Antibody (bsm-54280R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with NPHS2 (1A5) Monoclonal Antibody (bsm-54280R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of 293T cells with NPHS2 (1A5) Monoclonal Antibody (bsm-54280R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).