VALIDATION IMAGES
Lane 1: SK-BR-3 Cells; Lane 2: A549 Cells; Lane 3: Mouse Lung; Probed with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of HepG2 cells with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).