bsm-54282R [Primary Antibody]
PSMB8 (4A4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PSMB8

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5696

Swiss Prot: P28062

Source: Recombinant protein within human Proteasome 20S LMP7 aa 1-200

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 25


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: U937 Cells; Lane 2: A431 Cells; Probed with PSMB8 (4A4) Monoclonal Antibody (bsm-54282R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with PSMB8 (4A4) Monoclonal Antibody (bsm-54282R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with PSMB8 (4A4) Monoclonal Antibody (bsm-54282R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with PSMB8 (4A4) Monoclonal Antibody (bsm-54282R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with PSMB8 (4A4) Monoclonal Antibody (bsm-54282R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with PSMB8 (4A4) Monoclonal Antibody (bsm-54282R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with PSMB8 (4A4) Monoclonal Antibody (bsm-54282R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Daudi cells with PSMB8 (4A4) Monoclonal Antibody (bsm-54282R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).