VALIDATION IMAGES
Lane 1: Mouse Thymus; Lane 2: Mouse Lung; Lane 3: THP-1 Cells; Lane 4: HepG2 Cells; Probed with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of THP-1 cells with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Gelsolin (2G6) Monoclonal Antibody, Unconjugated (bsm-54290R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Human HepG2 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human Siha cell lysates probed with Gelsolin Monoclonal Antibody, Unconjugated (bsm-54290R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Blocking buffer: 5% NFDM/TBST <br>
Primary Ab dilution: 1:2000 <br>
Primary Ab incubation condition: 2 hours at <br>
room temperature <br>
Secondary Ab: Goat Anti-Rabbit IgG H&L <br>
(HRP) <br>
Lysate: Human plasma <br>
Protein loading quantity: 20 渭g <br>
Exposure time: 30 s <br>
Predicted MW: 81 kDa <br>
Observed MW: 81 kDa <br>