bsm-54290R [Primary Antibody]
Gelsolin Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Gelsolin

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2934

Swiss Prot: P06396

Source: Recombinant human Gelsolin protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 80


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Thymus; Lane 2: Mouse Lung; Lane 3: THP-1 Cells; Lane 4: HepG2 Cells; Probed with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of THP-1 cells with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Mouse Lung tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Gelsolin (2G6) Monoclonal Antibody, Unconjugated (bsm-54290R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Human HepG2 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human Siha cell lysates probed with Gelsolin Monoclonal Antibody, Unconjugated (bsm-54290R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Blocking buffer: 5% NFDM/TBST <br> Primary Ab dilution: 1:2000 <br> Primary Ab incubation condition: 2 hours at <br> room temperature <br> Secondary Ab: Goat Anti-Rabbit IgG H&L <br> (HRP) <br> Lysate: Human plasma <br> Protein loading quantity: 20 渭g <br> Exposure time: 30 s <br> Predicted MW: 81 kDa <br> Observed MW: 81 kDa <br>