bsm-54300R [Primary Antibody]
CD3G (2B11) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD3G

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 917

Swiss Prot: P09693

Source: Recombinant protein within C-terminal human CD3G

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed:8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 20


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Thymus; Probed with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Jurkat cells with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Thymus tissue lysates probed with CD3 gamma Monoclonal Antibody, Unconjugated (bsm-54300R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.