VALIDATION IMAGES
Lane 1: Mouse Testis; Lane 2: Rat Testis; Probed with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of PC-3M cells with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Galectin 8 (1A4) Monoclonal Antibody, Unconjugated (bsm-54301R) at 1:200 overnight at 4°C, DAB staining.