VALIDATION IMAGES
Lane 1: SiHa Cells; Probed with NPC1 (1B12) Monoclonal Antibody (bsm-54302R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with NPC1 (1B12) Monoclonal Antibody (bsm-54302R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with NPC1 (1B12) Monoclonal Antibody (bsm-54302R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with NPC1 (1B12) Monoclonal Antibody (bsm-54302R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with NPC1 (1B12) Monoclonal Antibody (bsm-54302R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with NPC1 (1B12) Monoclonal Antibody (bsm-54302R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with NPC1 (1B12) Monoclonal Antibody (bsm-54302R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of SH-SY5Y cells with NPC1 (1B12) Monoclonal Antibody (bsm-54302R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).