VALIDATION IMAGES
Lane 1: A431 Cells; Probed with Huntingtin (1F10) Monoclonal Antibody (bsm-54305R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Huntingtin (1F10) Monoclonal Antibody (bsm-54305R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Epididymis tissue incubated with Huntingtin (1F10) Monoclonal Antibody (bsm-54305R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Huntingtin (1F10) Monoclonal Antibody (bsm-54305R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of SH-SY5Y cells with Huntingtin (1F10) Monoclonal Antibody (bsm-54305R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with Huntingtin (1F10) Monoclonal Antibody (bsm-54305R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Huntingtin (1F10) Monoclonal Antibody, Unconjugated (bsm-54305R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Huntingtin (1F10) Monoclonal Antibody, Unconjugated (bsm-54305R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Huntingtin (1F10) Monoclonal Antibody, Unconjugated (bsm-54305R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Huntingtin (1F10) Monoclonal Antibody, Unconjugated (bsm-54305R) at 1:200 overnight at 4°C, DAB staining.