bsm-54306R
[Primary Antibody]
Cytochrome P450 17A1 Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Cytochrome P450 17A1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 1586
Swiss Prot: P05093
Source: Recombinant human Cytochrome P450 17A1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 57
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Hui Zhao. et al. Alleviating effects of selenium on fluoride-induced testosterone synthesis disorder and reproduction toxicity in rats. ECOTOX ENVIRON SAFE. 2022 Dec;247:114249Read more>>
- Qigang Fan. et al. Studying the effect of hyperoside on recovery from cyclophosphamide induced oligoasthenozoospermia. SYST BIOL REPROD MED. 2023 Aug 14Read more>>
VALIDATION IMAGES
IF(ICC) staining with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney incubated with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of SH=SY5Y cells with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytochrome P450 17A1 (2F7) Monoclonal Antibody, Unconjugated (bsm-54306R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytochrome P450 17A1 (2F7) Monoclonal Antibody, Unconjugated (bsm-54306R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytochrome P450 17A1 (2F7) Monoclonal Antibody, Unconjugated (bsm-54306R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cytochrome P450 17A1 (2F7) Monoclonal Antibody, Unconjugated (bsm-54306R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Adrenal gland lysates; Lane 2: Mouse Uterus lysates; Lane 3: Mouse Kidney lysates; Lane 4: Rat Testis lysates; Lane 5: Rat Adrenal gland lysates probed with Cytochrome P450 17A1 Monoclonal Antibody, Unconjugated (bsm-54306R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.