bsm-54308R [Primary Antibody]
STIP1 (2H5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: STIP1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10963

Swiss Prot: P31948

Source: Recombinant protein orresponding to human STIP1 aa 1-150

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Stress-induced-phosphoprotein 1 (STI1) functions as a co-chaperone for HSP70 and HSP90 during heat shock response. STI1 exists as either a monomer or a dimer, and this conformational flexibility facilitates its function in organizing HSP70/HSP90. HSP90 acts as an ATPase, and requires the recruitment of client proteins and proper conformation to function. STI1 acts as a "scaffold" for client protein recruitment to the relaxed, ADP-bound conformation of HSP90, thus suppressing ATP turnover during the loading phase and allowing proper function.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 63


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Testis; Lane 2: Rat Testis; Probed with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of K562 cells with STIP1 (2H5) Monoclonal Antibody (bsm-54308R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).