bsm-54319R [Primary Antibody]
CXCR5 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CXCR5

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 643

Swiss Prot: P32302

Source: Recombinant human CXCR5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 45


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Maria Chiara Trolese. et al. CXCL13/CXCR5 signalling is pivotal to preserve motor neurons in amyotrophic lateral sclerosis. Ebiomedicine. 2020 Dec;62:103097Read more>>
VALIDATION IMAGES

Lane 1: Daudi Cells; Probed with CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Daudi cells with CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCR5 (3B7) Monoclonal Antibody, Unconjugated (bsm-54319R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCR5 (3B7) Monoclonal Antibody, Unconjugated (bsm-54319R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCR5 (3B7) Monoclonal Antibody, Unconjugated (bsm-54319R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCR5 (3B7) Monoclonal Antibody, Unconjugated (bsm-54319R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Spleen lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human U937 cell lysates; Lane 4: Human MOLT-4 cell lysates; Lane 5: Human K562 cell lysates; Lane 6: Human A673 cell lysates probed with CXCR5 (3B7) Monoclonal Antibody, Unconjugated (bsm-54319R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.