VALIDATION IMAGES
Lane 1: Daudi Cells; Probed with CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Daudi cells with CXCR5 (3B7) Monoclonal Antibody (bsm-54319R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCR5 (3B7) Monoclonal Antibody, Unconjugated (bsm-54319R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCR5 (3B7) Monoclonal Antibody, Unconjugated (bsm-54319R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCR5 (3B7) Monoclonal Antibody, Unconjugated (bsm-54319R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CXCR5 (3B7) Monoclonal Antibody, Unconjugated (bsm-54319R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Spleen lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human U937 cell lysates; Lane 4: Human MOLT-4 cell lysates; Lane 5: Human K562 cell lysates; Lane 6: Human A673 cell lysates probed with CXCR5 (3B7) Monoclonal Antibody, Unconjugated (bsm-54319R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.