VALIDATION IMAGES
Lane 1: Mouse Thymus; Lane 2: MCF-7 Cells; Probed with PAK2 (6B4) Monoclonal Antibody (bsm-54323R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with PAK2 (6B4) Monoclonal Antibody (bsm-54323R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PAK2 (6B4) Monoclonal Antibody (bsm-54323R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with PAK2 (6B4) Monoclonal Antibody (bsm-54323R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with PAK2 (6B4) Monoclonal Antibody (bsm-54323R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with PAK2 (6B4) Monoclonal Antibody (bsm-54323R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of A431 cells with PAK2 (6B4) Monoclonal Antibody (bsm-54323R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).