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Host: Rabbit
Target Protein: FLOT1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 10211
Swiss Prot: O75955
Source: Recombinant protein within human Flotillin 1 aa 200-400
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Background:
Size: 100µL
Concentration: Lot Dependent
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC
Predicted Molecular Weight: 47
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: PC-3M Cells; Probed with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of HUVEC cells with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).