bsm-54330R [Primary Antibody]
H4C1 | Histone H4 (ac-K16) Recombinant Antibody
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Host: Rabbit

Target Protein: H4C1 | Histone H4 (ac-K16)

Modification Site: K16

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 121504

Swiss Prot: P62805

Source: Recombinant protein

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 are known to be acetylated on Lysine 5, 8, 12, and 16. Acetylations are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Hyper-acetylation of the histone tails is shown to neutralize positive charge and weakens histone-DNA and nucleosome-nucleosome interactions that destabilizes chromatin structure and increases the accessibility of DNA to various DNA-binding proteins.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC/IF(1:100-500)
IF(1:100-500)
ChIP(5μg/IP)
Flow-Cyt(1:50-100)

Predicted Molecular Weight: 11 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H4 (acetyl K16) Monoclonal Antibody, Unconjugated (bsm-54330R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Chromatin immunoprecipitation was performed using cross-linked chromatin from 5×10⁶ HeLa cells per IP, with 5 μg of bsm-54330R antibody or 5 μg of normal rabbit IgG(negative control bs-0295P), and 20 μL of Protein A/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-CDS and RPL30 exon 4 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.


C2C12 (M) treated with Trichostatin A (TSA) (1μM) for 18 h, HeLa (H) treated with Trichostatin A (TSA) (1μM) for 18 h, 25 μg total protein per lane of cell lysates (see on figure) probed with Histone H4 (acetyl K16) monoclonal antibody, unconjugated (bsm-54330R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.