bsm-54330R-PE

DATASHEET

Host: Rabbit

Target Protein: H4C1 | Histone H4 (ac-K16)

Modification Site: K16

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 121504

Swiss Prot: P62805

Source: Recombinant protein

Purification: purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 are known to be acetylated on Lysine 5, 8, 12, and 16. Acetylations are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Hyper-acetylation of the histone tails is shown to neutralize positive charge and weakens histone-DNA and nucleosome-nucleosome interactions that destabilizes chromatin structure and increases the accessibility of DNA to various DNA-binding proteins.

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC/IF(1:100-500)
IF(1:100-500)
ChIP(5μg/IP)
Flow-Cyt(1:50-100)

Predicted Molecular Weight: 11 kDa

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.