VALIDATION IMAGES
Lane 1: SiHa Cells; Probed with CD146 (1G3) Monoclonal Antibody (bsm-54332R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CD146 (1G3) Monoclonal Antibody (bsm-54332R) at 1:200 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CD146 (1G3) Monoclonal Antibody (bsm-54332R) at 1:200 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with CD146 (1G3) Monoclonal Antibody (bsm-54332R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CD146 (1G3) Monoclonal Antibody (bsm-54332R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with CD146 (1G3) Monoclonal Antibody (bsm-54332R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of HUVEC cells with CD146 (1G3) Monoclonal Antibody (bsm-54332R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).