VALIDATION IMAGES
Lane 1: Human Colon; Probed with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of HUVEC cells with CREB1 (Ser133) (5F2) Monoclonal Antibody (bsm-54334R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).