bsm-54337R [Primary Antibody]
ACTR3 (1A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ACTR3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10096

Swiss Prot: P61158

Source: Recombinant protein of C-terminal human Arp3.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A431 Cells; Lane 2: Mouse Placenta; Lane 3: Mouse Thymus; Probed with ACTR3 (1A1) Monoclonal Antibody (bsm-54337R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with ACTR3 (1A1) Monoclonal Antibody (bsm-54337R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with ACTR3 (1A1) Monoclonal Antibody (bsm-54337R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with ACTR3 (1A1) Monoclonal Antibody (bsm-54337R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with ACTR3 (1A1) Monoclonal Antibody (bsm-54337R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with ACTR3 (1A1) Monoclonal Antibody (bsm-54337R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with ACTR3 (1A1) Monoclonal Antibody (bsm-54337R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with ACTR3 (1A1) Monoclonal Antibody (bsm-54337R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of HL-60 cells with ACTR3 (1A1) Monoclonal Antibody (bsm-54337R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).