VALIDATION IMAGES
Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with CYP26A1 (11A1) Monoclonal Antibody (bsm-54341R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with CYP26A1 (11A1) Monoclonal Antibody (bsm-54341R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: HepG2 Cells; Probed with CYP26A1 (11A1) Monoclonal Antibody (bsm-54341R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human A549 cell lysates probed with CYP26A1 Monoclonal Antibody, Unconjugated (bsm-54341R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CYP26A1 Monoclonal Antibody, Unconjugated (bsm-54341R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CYP26A1 Monoclonal Antibody, Unconjugated (bsm-54341R) at 1:200 overnight at 4°C, DAB staining.