bsm-54343R [Primary Antibody]
Gephyrin (7A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Gephyrin

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10243

Swiss Prot: Q9NQX3

Source: Recombinant protein within human Gephyrin aa 1-100.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

The sub-membraneous region at the postsynaptic membrane contains a number of proteins critical for receptor targeting. Gephyrin is a microtubule-associated protein highly expressed in brain and localized to neuronal postsynaptic membranes. Gephyrin is essential for the postsynaptic localization of the inhibitory glycine receptor and is thought to anchor the receptor to subsynaptic microtubules. The protein is expressed in most mammalian tissues with predominant expression in brain. At least five additional splice variants of Gephyrin ranging in molecular weight have been identified in rat and human brain tissue.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 80


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Gephyrin (7A1) Monoclonal Antibody (bsm-54343R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Gephyrin (7A1) Monoclonal Antibody (bsm-54343R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: SK-BR-3 Cells; Lane 2: Rat Kidney; Probed with Gephyrin (7A1) Monoclonal Antibody (bsm-54343R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.