bsm-54345R [Primary Antibody]
ELMO1 (12A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ELMO1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 9844

Swiss Prot: Q92556

Source: Recombinant protein within human ELMO1 aa 500 to 700.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 84


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Spleen; Lane 2: Jurkat Cells; Probed with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Spleen tissue incubated with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Spleen tissue incubated with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of SH-SY5Y cells with ELMO1 (12A1) Monoclonal Antibody (bsm-54345R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).