bsm-54347R [Primary Antibody]
USP9x (11G1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: USP9x

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8239

Swiss Prot: Q93008

Source: Recombinant protein within human USP9X aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-48'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Involved in axonal growth and neuronal cell migration. Regulates cellular clock function by enhancing the protein stability and transcriptional activity of the core circadian protein ARNTL/BMAL1 via its deubiquitinating activity.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 292


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SiHa Cells; Lane 2: A549 Cells; Lane 3: 293 Cells; Lane 4: Mouse Colon; Probed with USP9x (11G1) Monoclonal Antibody (bsm-54347R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with USP9x (11G1) Monoclonal Antibody (bsm-54347R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with USP9x (11G1) Monoclonal Antibody (bsm-54347R) at 1:100 in F9 Cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with USP9x (11G1) Monoclonal Antibody (bsm-54347R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with USP9x (11G1) Monoclonal Antibody (bsm-54347R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of 293T cells with USP9x (11G1) Monoclonal Antibody (bsm-54347R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).


Paraformaldehyde-fixed and paraffin-embedded Mouse Fallopian Tube tissue incubated with USP9x (11G1) Monoclonal Antibody (bsm-54347R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.