VALIDATION IMAGES
Lane 1: SiHa Cells; Probed with Pumilio 1 (7B2) Monoclonal Antibody (bsm-54349R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Pumilio 1 (7B2) Monoclonal Antibody (bsm-54349R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Pumilio 1 (7B2) Monoclonal Antibody (bsm-54349R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Pumilio 1 (7B2) Monoclonal Antibody (bsm-54349R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with Pumilio 1 (7B2) Monoclonal Antibody (bsm-54349R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with Pumilio 1 (7B2) Monoclonal Antibody (bsm-54349R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with Pumilio 1 (7B2) Monoclonal Antibody (bsm-54349R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of 293T cells with Pumilio 1 (7B2) Monoclonal Antibody (bsm-54349R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).