bsm-54351R [Primary Antibody]
Syntrophin alpha 1 (2A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SNTA1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6640

Swiss Prot: Q13424

Source: Recombinant protein within human Syntrophin alpha 1 aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-bisphosphate.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 54


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SiHa Cells; Probed with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Skeletal Muscle tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of LOVO cells with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).