bsm-54358R [Primary Antibody]
SAE1 (1F1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SAE1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10055

Swiss Prot: Q9UBE0

Source: Recombinant protein within human SAE1 aa 150-300.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 38


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SiHa Cells; Lane 2: K562 Cells; Lane 3: 293 Cells Probed with SAE1 (1F1) Monoclonal Antibody (bsm-54358R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of A549 cells with SAE1 (1F1) Monoclonal Antibody (bsm-54358R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).