bsm-54361R [Primary Antibody]
NDUFB8 (25B3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NDUFB8

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4714

Swiss Prot: O95169

Source: Recombinant protein within human NDUFB8 aa 30-130.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 17


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: 293 Cells; Lane 2: A549 Cells; Lane 3: Mouse Heart; Lane 4: Rat Spleen; Probed with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of 293T cells with NDUFB8 (25B3) Monoclonal Antibody (bsm-54361R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).