bsm-54366R [Primary Antibody]
VAMP3 (8A3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VAMP3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 9341

Swiss Prot: Q15836

Source: Synthetic peptide conjugated to KLH within N-terminal human VAMP3/Cellubrevin.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP3 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. VAMP3 is involved in vesicular transport from the late endosomes to the trans-Golgi network.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 11


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A549 Cells; Lane 2: 293T Cells; Probed with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of SiHa cells with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Hippocampus tissue incubated with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.