bsm-54379R [Primary Antibody]
Tetranectin (4B3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CLEC3B

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7123

Swiss Prot: P05452

Source: Recombinant protein within human Tetranectin aa 100-250.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Tetranectin is a homotrimeric glycoprotein present in plasma and various tissue locations that binds to calcium, heparin, and plasminogen kringle 4. Tetranectin may play a prominent role in tissue remodeling as well as in the regulation of proteolytic processes via its binding and indirect activation of plasminogen. Tetranectin is found in the extracellular matrix (ECM) of certain carcinomas, but is not present in the ECM of normal tissues. Extracellular proteolysis is an important factor in the ability of malignant cells to penetrate normal tissues and metastasize. Decreased plasma tetranectin or increased tetranectin in stroma of cancers correlates with cancer progression and a grim prognosis. Tetranectin may also influence cancer growth by altering activities of plasminogen or the plasminogen fragment, angiostatin which inhibits tumor cell proliferation.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 22


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Rat Skin; Lane 2: Human Skin; Probed with Tetranectin (4B3) Monoclonal Antibody (bsm-54379R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Tetranectin (4B3) Monoclonal Antibody (bsm-54379R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Tetranectin (4B3) Monoclonal Antibody (bsm-54379R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with Tetranectin (4B3) Monoclonal Antibody (bsm-54379R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Tetranectin (4B3) Monoclonal Antibody (bsm-54379R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.