bsm-54382R [Primary Antibody]
RanGAP1 (2A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RanGAP1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5905

Swiss Prot: P46060

Source: Recombinant protein within human RanGAP1 aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

The small Ras related protein Ran, also called TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The abundant GEF RCC1 (regulator of chromosome condensation 1) increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. Ran BP2 has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 64


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: MCF-7 Cells; Lane 2: SiHa Cells; Lane 3: 293 Cells; Probed with RanGAP1 (2A1) Monoclonal Antibody (bsm-54382R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with RanGAP1 (2A1) Monoclonal Antibody (bsm-54382R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with RanGAP1 (2A1) Monoclonal Antibody (bsm-54382R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with RanGAP1 (2A1) Monoclonal Antibody (bsm-54382R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with RanGAP1 (2A1) Monoclonal Antibody (bsm-54382R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with RanGAP1 (2A1) Monoclonal Antibody (bsm-54382R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of A431 cells with RanGAP1 (2A1) Monoclonal Antibody (bsm-54382R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).