bsm-54385R [Primary Antibody]
Lipin 1 (2D10) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LPIN1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 23175

Swiss Prot: Q14693

Source: Recombinant protein within human Lipin 1 aa 50-250.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 98


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: K562 Cells; Lane 2: SH-SY5Y Cells; Lane 3: Mouse Lung; Probed with Lipin 1 (2D10) Monoclonal Antibody (bsm-54385R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Lipin 1 (2D10) Monoclonal Antibody (bsm-54385R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Lipin 1 (2D10) Monoclonal Antibody (bsm-54385R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with Lipin 1 (2D10) Monoclonal Antibody (bsm-54385R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Lipin 1 (2D10) Monoclonal Antibody (bsm-54385R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Skeletal Muscle tissue incubated with Lipin 1 (2D10) Monoclonal Antibody (bsm-54385R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of PC-3M cells with Lipin 1 (2D10) Monoclonal Antibody (bsm-54385R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).